Elucidation of a masked repeating structure of the O-specific polysaccharide of the halotolerant soil bacteria Azospirillum halopraeferens Au4

• Elena N. Sigida,
• Yuliya P. Fedonenko,
• Alexander S. Shashkov,
• Nikolay P. Arbatsky,
• Evelina L. Zdorovenko,
• Svetlana A. Konnova,
• Vladimir V. Ignatov and
• Yuriy A. Knirel

Beilstein J. Org. Chem. 2016, 12, 636–642, doi:10.3762/bjoc.12.62

• hydrolysis of the lipopolysaccharide isolated by the phenol–water extraction from the halotolerant soil bacteria Azospirillum halopraeferens type strain Au4. The polysaccharide was studied by sugar and methylation analyses, selective cleavages by Smith degradation and solvolysis with trifluoroacetic acid

• residue (~65%), are shown in italics. Keywords: Azospirillum halopraeferens; bacterial polysaccharide structure; lipopolysaccharide; O-specific polysaccharide; Smith degradation; triflic acid solvolysis; Introduction Rhizobacteria of the genus Azospirillum are isolated from a wide variety of

• above), elimination of the side-chain Glc and cleavage of the linkage of the destroyed 2-substituted Xyl were expected. Fractionation of the Smith degradation products by TSK HW-40 (S) gel-permeation chromatography resulted in a mixture of the expected oligosaccharides 1 and 2 and higher molecular mass